Biotinylated Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human MSLN is greater than 95% as determined by SEC-HPLC.
Use Biotinylated Human MSLN-hFc-Avi protein to detect the expression rate of Anti-MSLN-CAR positive cell. 293T cells and anti-MSLN CAR-293T cells were incubated with Biotinylated human MSLN-hFc-Avi Tag. Non-transfected 293T cells and Fc-labeled protein control were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human MSLN.
Immobilized Anti-MSLN Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA.
Supplier Page from Kactusbio Inc. for Biotinylated Human MSLN/Mesothelin Protein (MSL-HM580B)